Inst ToolsInst ToolsInst Tools
  • Courses
  • Automation
    • PLC
    • Control System
    • Safety System
    • Communication
    • Fire & Gas System
  • Instrumentation
    • Design
    • Pressure
    • Temperature
    • Flow
    • Level
    • Vibration
    • Analyzer
    • Control Valve
    • Switch
    • Calibration
    • Erection & Commissioning
  • Interview
    • Instrumentation
    • Electrical
    • Electronics
    • Practical
  • Q&A
    • Instrumentation
    • Control System
    • Electrical
    • Electronics
    • Analog Electronics
    • Digital Electronics
    • Power Electronics
    • Microprocessor
  • Request
Search
  • Books
  • Software
  • Projects
  • Process
  • Tools
  • Basics
  • Formula
  • Power Plant
  • Root Cause Analysis
  • Electrical Basics
  • Animation
  • Standards
  • 4-20 mA Course
  • Siemens PLC Course
Reading: Liquid Chromatography Questions & Answers
Share
Font ResizerAa
Inst ToolsInst Tools
Font ResizerAa
  • Courses
  • Design
  • PLC
  • Interview
  • Control System
Search
  • Courses
  • Automation
    • PLC
    • Control System
    • Safety System
    • Communication
    • Fire & Gas System
  • Instrumentation
    • Design
    • Pressure
    • Temperature
    • Flow
    • Level
    • Vibration
    • Analyzer
    • Control Valve
    • Switch
    • Calibration
    • Erection & Commissioning
  • Interview
    • Instrumentation
    • Electrical
    • Electronics
    • Practical
  • Q&A
    • Instrumentation
    • Control System
    • Electrical
    • Electronics
    • Analog Electronics
    • Digital Electronics
    • Power Electronics
    • Microprocessor
  • Request
Follow US
All rights reserved. Reproduction in whole or in part without written permission is prohibited.
Inst Tools > Blog > Multiple Choice Questions > Liquid Chromatography Questions & Answers

Liquid Chromatography Questions & Answers

Last updated: November 27, 2021 5:37 pm
Editorial Staff
Multiple Choice Questions
No Comments
Share
5 Min Read
SHARE

Liquid Chromatography Questions & Answers

1. Which of the following is the disadvantage of reciprocating pump used in liquid chromatography?

a) Produces pulsed flow
b) Corrosive components
c) Does not have small hold-up value
d) Does not have moderate flow rate

Answer: a

Explanation: The disadvantage of reciprocating pump used in liquid chromatography is that it produces pulsed flow. Therefore, the flow must be damped before it affects the column.

2. Which of the following is not a disadvantage of Pneumatic pumps used in liquid chromatography?

a) Pulsed output
b) Dependent on solvent viscosity
c) Dependent on back pressure
d) Inconvenient for solvent gradient elution

Answer: a

Explanation: Pneumatic pumps provide pulse free output. They are inexpensive.

3. Which of the following is not a desired characteristic of pulse dampers or flow smootheners used in liquid chromatography?

a) Easy mobile phase change over
b) Constant flow must be maintained
c) Should be effective at low system pressure
d) Maximal dead volume

Answer: d

Explanation: Pulse dampers are used to avoid variations in flow. They must have minimal dead volume.

4. Which of the following will improve the efficiency of separation process in liquid chromatography?

a) Increase in sample size, increase in column diameter
b) Reduction in sample size, increase in column diameter
c) Increase in sample size, reduction in column diameter
d) Reduction in sample size, reduction in column diameter

Answer: d

Explanation: Reduction in sample size and reduction in column diameter will improve the efficiency of separation process in liquid chromatography. The effect of uneven flow will also be reduced.

5. Which of the following are the practical problems that arise due to the decrease in column diameter?

a) Requirement of large particle size and high pressure drop
b) Requirement of large particle size and low pressure drop
c) Requirement of small particle size and high pressure drop
d) Requirement of small particle size and low pressure drop

Answer: c

Explanation: The practical problems that arise due to decrease in column diameter are requirement of small particle size and high pressure drop. But, it increases column efficiency.

6. Which of the following is not true about guard column used in liquid chromatography?

a) It filters particles that clog the separation column
b) It extends the lifetime of separation column
c) It allows particles that cause precipitation upon contact with stationary or mobile phase
d) The size of packing varies with the type of protection needed

Answer: c

Explanation: Guard column removes particles that cause precipitation upon contact with stationary or mobile phase. It is placed before the separation column.

7. Which of the following columns are not used in liquid or high performance liquid chromatography?

a) Analytical column
b) Separation column
c) Guard column
d) Capillary column

Answer: d

Explanation: Capillary column is used in gas chromatography. Analytical column is also known as separation column.

8. Which of the following is not a Column-type Liquid chromatography?

a) Gel permeation
b) Ion exchange
c) Liquid-solid
d) Paper

Answer: d

Explanation: Paper chromatography is not a column-type chromatography. It makes use of strips of hollow cylinders of filter paper.

9. Which of the following is not true about radial compression column when compared to standard separation column?

a) Internal diameter decreases
b) Overall operating pressure decreases
c) Analysis time decreases
d) Solvent flow increases

Answer: a

Explanation: Radial compression columns have a wider diameter than standard separation column. The cartridges used are of low cost.

10. Which of the following is not true about narrow bore column when compared to standard columns?

a) Internal diameter decreases
b) Volumetric flow decreases
c) Solvent cost is saved
d) Detector response time increases

Answer: d

Explanation: Detector response time increases in narrow bore columns. High purity solvents must be used in these columns.

11. Which of the following types of liquid chromatography uses immobilized biochemical as stationary phase?

a) Ion exchange chromatography
b) Exclusion chromatography
c) Affinity chromatography
d) Gel permeation chromatography

Answer: c

Explanation: In Affinity chromatography, immobilized biochemical is used as stationary phase. It is used to separate one or few solutes from hundreds of unretained solutes.

Don't Miss Our Updates
Be the first to get exclusive content straight to your email.
We promise not to spam you. You can unsubscribe at any time.
Invalid email address
You've successfully subscribed !

Continue Reading

MCQ on Measurement and Instrumentation
Top 100 Control Systems Objective Questions & Answers
Hydraulic Systems Objective Questions
Z & S Domain Relationship
Infrared Gas Analyzers Questions & Answers
State Variables & Linear Discrete Time Systems
Share This Article
Facebook Whatsapp Whatsapp LinkedIn Copy Link
Share
Leave a Comment

Leave a Reply Cancel reply

Your email address will not be published. Required fields are marked *

Stay Connected

128.3kFollowersLike
69.1kFollowersFollow
210kSubscribersSubscribe
38kFollowersFollow

Categories

Explore More

Approximation of Higher-Order Systems by Lower Order
Liapunov’s Stability Criterion – Part I
Nuclear Magnetic Resonance Spectrometer Questions & Answers
Hydrogen and Glass Electrodes Questions & Answers
Radiofrequency Mass Spectrometer
Linear Approximation of the Non-Linear System MCQ
Effect of Adding a Zero to a System – II
Chromatography Questions & Answers

Keep Learning

Advances in Control System

Adaptive Control

Control Systems Multiple Choice Questions

Ion Analyzers Questions and Answers

Special Designs of Ion Selective Electrodes Questions & Answers

Stability in Frequency Domain

Assessment of Relative Stability Using Nyquist Criterion

Chromatography Questions & Answers

Detection System of Liquid Chromatography Questions & Answers

Ion Analyzers Questions and Answers

Ion Selective Electrodes Questions and Answers

Feedback Characteristics of Control Systems

Controller Components – Part 1 Objective Questions

Frequency Response MCQ

All-pass & Minimum-phase Systems

Learn More

Three-Phase Wye Wiring System

4-Wire, Three-Phase Wye Wiring System

Pressure Safety Valves Functional Testing

Pressure Safety Valves Functional Testing

Generator Protection Interview Questions

Generator Protection Interview Questions & Answers

Digital Transducers Objective Questions

Digital Transducers Objective Questions

Power Systems Questions & Answers

Power Systems MCQ Series 20

flow-meter-accuracy-calculation

Impact of Flow Meter Accuracy

DeltaV System to Excel

How to Export Data from DeltaV System to Excel

PLC Conversion Program

How to use Converters in PLC

Menu

  • About
  • Privacy Policy
  • Copyright

Quick Links

  • Learn PLC
  • Helping Hand
  • Part Time Job

YouTube Subscribe

Follow US
All rights reserved. Reproduction in whole or in part without written permission is prohibited.
Welcome Back!

Sign in to your account

Username or Email Address
Password

Lost your password?